T4 ligasi promega

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August undefined, 2024

Web• 1 μL of T4 DNA ligase (Promega) • x μL water up to 20 μL total. *BsaI-HF is optimized for different buffer conditions and doesn't work well ** Promega T4 Ligase buffer has been shown to offer the highest activity for BsaI enzyme. Other T4 ligase enzymes will work, but Promega enzyme with Promega buffer is highest efficiency. WebPromega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 · www.promega.com Part# TM042 Printed in USA. Page 6 Revised 5/07 ScaI 1890 ori pGEM -T Easy Vector (3015bp) Ampr SpeI EcoRI NotI BstZI PstI SalI NdeI SacI BstXI NsiI T7 XmnI 2009 NaeI 2707

Promega pGEM™-T and pGEM™-T Easy Vector Systems - Fisher Sci

WebT4DNA Ligaseとユニークな2X Rapid Ligation Bufferを組合せています。連結されたDNAはトランスフォーメーションをする前に精製する必要がありません。また、特製の2X Rapid Ligation Bufferは使用前にATPやMg2+を加える必要はありません。迅速: 突出（粘着）末端なら5分間、平滑末端なら15分間効率的: 他社製品に較べ優れたライゲーション効率便 … kroger locations in troy mi

T4 DNA Ligase Ligase DNA Ligation - Promega

WebT4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended … WebT4 DNA Ligase Joins two dsDNA strands with cohesive or blunt ends by using the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. M1801, M1804, M1794 … WebSynthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster. In Current Protocols on 1 February 2024 by Venken, K. J. T., Matinyan, N., et al.. kroger locations in the southwest us

T4 DNA Ligase Buffer - Thermo Fisher Scientific

New England Biolabs (UK) Ltd - T4 DNA Ligase

WebSynthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster. In … WebPlease Enquire. T4 DNA Ligase. 1–3u/µl/100u (Weiss units) ¥ 9,000. T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´ … map of hobe sound fl areaWebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. map of hobgood nc

"WebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5′-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from … " - T4 ligasi promega

T4 ligasi promega

WebDec 1, 2024 · 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes. Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: 800-356-9526 Fax: 800-356-1970 Company Profile Website: http://www.promega.com Citations (64) WebLigation Calculator. This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. Ligation. Tutorials. Insert DNA length. Vector DNA length. Vector DNA mass. Required insert DNA mass.

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WebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as … WebDescription. The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf (+) Vector with Eco R V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid. 2X Rapid Ligation Buffer allows reactions to be completed in one hour ...

WebPromega T4 DNA Ligase Print Promega T4 DNA Ligase For ligation of DNA ends. $69.00 - $294.50 Specifications. Format: Liquid: Color: Colorless: Components: 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP: Purity >90%: pH: 7.4: View More Specs. Products 3 ... WebT4 DNA Ligase Buffer Pack 1.5ml Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. Formulation: 300mM Tris-HCl (pH 7.8), 100mM MgCl 2, 100mM DTT, 10mM ATP. Specifications What's in the box? SDS Search for SDS

WebT4 RNA Ligase 500u ¥ 24,200 T4 RNA Ligaseは、ATPに依存して、1本鎖RNAまたはDNAの分子間および分子内の連結を行い、1本鎖RNAまたはDNAの5'-末端のリン酸基と3'-末端の水酸基を結合します。同様に、1本鎖RNAに [5'- 32 P]nucleoside3',5'-bis (phosphate)を付加する反応も行います。本酵素はRNase Iを欠如する組換えE.coli CA4株から精製し … WebMay 15, 2024 · ATP-binding cassette (ABC) transporters are a superfamily of proteins that transport nutrient substances and secondary metabolites through cell membranes. They also act as an uptake system for N,N′-diacetylchitobiose (GlcNAc)2 in Streptomyces coelicolor. (GlcNAc)2 is an important inducer of chitinase. However, whether the ABC …

WebPromega T4 DNA Ligase. For ligation of DNA ends. Manufacturer: Promega M1804. View more versions of this product. Catalog No. PR-M1804. $278.50 / Each of 1. Qty Check Availability. Add to cart. Description.

WebLa fusariose vasculaire du cyclamen est une maladie causée par le champignon tellurique Fusarium oxysporum f. sp. cyclaminis. Elle est considérée comme l’une des maladies les plus graves du cyclamen et se traduit par des pertes … map of hobe sound floridaWeb2X Rapid Ligation Buffer. 1.5ml. Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. Ligate DNA inserts into plasmid vectors in as little as 15 minutes. No purification necessary prior to transformation of ... map of hochatown oklahomaWebT4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, … kroger locations map 2020WebJul 8, 2024 · The PCR fragment is directly ligated to the T-tailed plasmid vector with no need for further enzymatic treatment other than the action of T4 DNA ligase. If desired, the insert can then be easily transferred from the T vector to other plasmids using the restriction sites present in the T vector. map of hoburne cotswoldWeb小鼠精胺氧化酶基因SMO启动子的结构和功能研究.pdf kroger locations mapWebWe regularly use DH5a cells for transformation, and we have good success with transformation following a 12-14 hour ligation at 4oC with the T4 ligase. We do not inactivate the ligase. We do not ... kroger locations knoxville tnWeb原装现货大鼠骨成型蛋白受体Ⅱ进口供应(BMPR-Ⅱ)ELISA Kit免费代测，北京报价样品收集、处理及保存方法 1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。 2. 血浆：EDTA、柠檬 … map of hochatown oklahoma area