Webdevelopment of high-fidelity Cas13 variants with markedly reduced collateral effects by mutagenesis and demonstrated the feasibility of hfCas13 for efficient on-target RNA degradation with almost no Web29 de nov. de 2024 · They discovered a family of CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), …
A high-fidelity RNA-targeting Cas13 restores paternal Ube3a …
Web17 de jan. de 2024 · High-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno-associated-virus-mediated somatic cell targeting. Web18 de fev. de 2024 · A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Author links open overlay panel Jinhui Li 1 6, Zhixin Shen 1 6, Yajing Liu 1 6, Zixiang Yan 3 6, Yuanhua Liu 1 6, Xiang Lin 4, Junjie Tang 1, Ruimin Lv 1, Guannan Geng 1, Zhi-Qi Xiong 1, Changyang … danny litwhiler baseball
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Web16 de ago. de 2024 · The study, published in Nature Biotechnology in the paper, “ High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects, ” demonstrates the feasibility of hfCas13 for efficient on-target RNA degradation with almost no collateral damage in mammalian cells and animals. Concerns have been raised over … Web15 de mar. de 2024 · High-Fidelity CRISPR/Cas13a trans-Cleavage-Triggered Rolling Circle Amplified DNAzyme for Visual Profiling of MicroRNA. ... CRISPR/Cas13-Based … Web18 de fev. de 2024 · A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Author links open overlay panel Jinhui Li 1 6, Zhixin Shen 1 6, Yajing Liu 1 6, Zixiang Yan 3 6, Yuanhua Liu 1 6, Xiang Lin 4, Junjie Tang 1, Ruimin Lv 1, Guannan Geng 1, Zhi-Qi Xiong 1, Changyang … danny litwhiler